FBS was from Gibco. Atorvastatin (sodium salt, dissolved in Tris-borate, pH 8) was from Pfizer. C3 exoenzyme was from Calbiochem, and FuGENE 6 transfection reagent was from Roche. The remaining ...

Here we characterize the interaction of 14-3-3 with a nonphosphorylated protein ligand, the ADP-ribosyltransferase Exoenzyme S (ExoS) from Pseudomonas aeruginosa. By using affinity chromatography and ...

The heart is a never-stopping engine that relies on a formidable pool of mitochondria to generate energy and propel pumping. Because dying cardiomyocytes cannot be replaced, this high metabolic rate ...

Limitation of soil microorganisms by key nutrients such as nitrogen (N) and phosphorus (P) controls ecosystem processes, including soil carbon cycling. Thus, it is fundamental to understand patterns ...

In the present study, we used a small pool screening approach to identify novel 14‐3‐3‐binding proteins. One of the molecules that we identified functions as a transcriptional regulator in mammalian ...

Across animal species, from invertebrates to vertebrates, sleep behavior is regulated in a homeostatic manner (1–3).A homeostatic sleep need reflects the “integral” of the quantity and quality of ...