Accurate quantification of RNA in situ hybridization (RNA-ISH) signals is critical for understanding spatial gene expression, particularly at the single-cell level. Traditional methods using ...

Public substantial RNA-sequencing data of cardiac circRNAs were utilized ... a regulatory mechanism on transcription mediated by N6-methyladenosine-circRNA-histone methylation in pathological cardiac ...

The RNA m5C methylation modification, which adds a donor active methyl ... cell viability was assessed by the Cell Counting Kit-8 (CCK-8; Meilun Bio, China) and MTT assay kit (Meilun Bio, China) ...

Mass spectrometry has become the most powerful method for the large-scale discovery and quantification of histone PTMs, and has already produced a rich catalog of novel modifications, many of ...

This cover highlights the article Slik sculpts the plasma membrane into cytonemes to control cell-cell communication by Basile Rambaud, Mathieu Joseph, David R. Hipfner, Sébastien Carréno and ...

A Snakemake 8 workflow for end-to-end processing, quantification, and annotation of gene expression for RNA-seq experiments, starting from single- or paired-end reads within raw/unaligned/unmapped ...

Next, the p-values of m6A-related genes in the experimental and control groups were determined using Wilcoxon test and visualized in the ggplot2 package. Differentially expressed genes of m6A ...

This study clearly shows that m6A modification on mRNA regulates immune responses in the intestine during rotavirus infection. The authors further show the mechanisms how m6A modification is regulated ...

The paper addresses an important unresolved mechanism of antibiotic resistance caused by a RNA-modifying enzyme Cfr, a protein that confers resistance to multiple ribosome-targeting antibiotics due to ...