An artificial chimaera of crRNA and tracrRNA, the two bacterial RNA components that direct Cas9 to DNA sequences for cleavage. The first ∼20 bp of sgRNA (or crRNA) are variable and complementary to ...

Guide sequence (gRNA) shown in the bottom gray box was cloned into vector to express gRNA guiding Cas9 exonuclease to the targeted protospacer adjacent motif sequence. (B) Next generation sequencing ...

We demonstrate that these ancient forms were much more flexible in their guide RNA and protospacer-adjacent motif requirements compared with modern-day Cas9 enzymes. Furthermore, anCas portrays a ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ...

The Cas9 protein is the most widely used by scientists. This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it ...

Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. Molecular logic gates, as biomolecule-based computational systems, are highly suitable ...

Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City 700000, Vietnam Vietnam National University, Ho Chi Minh City ...

Also, due to their diverse nature of functionality, cas9 endoribonuclease can be easily reprogrammed with the help of guide RNAs, showing unprecedented potential and significance for gene editing in ...