With CRISPR-Cas9, the crRNA/tracrRNA sequence or an artificial guide RNA indicate where the DNA can be cut. It is relatively easy for scientists to produce different sequence variants from RNA ...
Pyogenes, the Edit-R platform comprises the three components required for gene editing in mammalian cells: (1) A plasmid encoding Cas9 nuclease, (2) a chemically-synthesized trans-activating RNA ...
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