Steps 1–14, construction of CRISPR-Cas9 vector for CRIS-PITCh (v2): 1 week Steps 15–25, construction of CRIS-PITCh (v2) donor vector: 3 d ...

Interestingly, nano-vectors loaded with Cas9 RNP can directly enter the nucleus to perform CRISPR/Cas9-mediated genome editing. A poly (d,l-lactide-co-glycolide) (PLGA)-based nano-vector for Cas9 ...

In addition, first-generation CRISPR systems (Cas9 and Cas12a) are too large for efficient in vivo delivery via a single adeno-associated viral (AAV) vector. Now, new preclinical research ...

More information: Killian S. Hanlon et al. High levels of AAV vector integration into CRISPR-induced DNA breaks, Nature Communications (2019). DOI: 10.1038/s41467-019-12449-2 ...

Conventional CRISPR nucleases, including Cas9 and Cas12a, exceed the packaging limits of a single AAV vector, necessitating dual-AAV strategies that reduce efficiency. Smaller CRISPR systems such ...

Vector offers custom design and manufacturing services for AAV and adenovirus, as well as catalog vectors for overexpression, shRNA, miRNA, and CRISPR. Vector’s products have been referenced in ...

The well-established platform allows for the ability to support the exacting viral vector manufacturing needs of the NCH NK cells created on the hospital's patented universal-donor NK cell platform.