and then tested several voltages to find one that delivers the CRISPR/Cas9 components inside the cell without causing it harm. In addition to faster throughput, the researchers' electroporation ...

Since electroporation can damage some cells, and other cells appear to resist it altogether, the strategy could provide a promising alternative for CRISPR delivery. The Feldan shuttle is inspired ...

This article offers strategies to optimize and improve CRISPR-Cas9 delivery even in hard-to ... An attractive alternative is electroporation-based Nucleofection™ technology.

Techniques such as electroporation have facilitated efficient ... thereby demonstrating the evolving landscape of CRISPR-Cas delivery methods and their potential to overcome current limitations.

Among them, electroporation is the most commonly ... Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework.

Efficient and reliable delivery of DNA ... stem and progenitor cells (HSPCs) after CRISPR-Cas9 transfection utilizing the MaxCyte ® electroporation technology to address this issue.

and protein molecules required for CRISPR to function, resulting in predictable delivery. Gold nanoparticles can also be incredibly small and are well suited for slipping into cells efficiently ...

The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation ... One challenge has been the efficient and consistent delivery of a range of gene-editing tools into human ...

(2) Researchers deliver CRISPR system components using different methods, including conjugation, transformation by heat shock or electroporation ... in it still have many challenges ahead. DNA ...