We next aimed to verify the dCas9-EGFP signals with FISH using probes targeting repetitive sequences so that a single FISH probe could access many sites. We used another two sgRNA sequences ...

CRISPR-dCas9 and sgRNA – RNA origami fusion molecules Researchers from Aarhus University publish on RNA origami enabling applications in synthetic biology Original Source ...

The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...

(Courtesy of Stanley Qi) CRISPRKit works in a similar way, though it employs dCas9, or “dead” Cas9, a deactivated version of the Cas9 enzyme unable to cut DNA. “Whereas CRISPR-Cas9 is ...

It's CRISPR, without the cut. The Horizon CRISPRi system uses a deactivated Cas9 nuclease (dCas9) fused to our proprietary SALL1-SDS3 repressor construct to block transcription of target genes, ...