Over the past decade, lipid nanoparticles (LNPs) have become a cornerstone in the field of nucleic acid delivery, with applications extending from vaccines ...

This version of CRISPR uses an engineered protein called dCas9, together with an RNA molecule that acts as a mailing address for a particular site in the genome. This enables the dCas9-RNA complex ...

(Courtesy of Stanley Qi) CRISPRKit works in a similar way, though it employs dCas9, or “dead” Cas9, a deactivated version of the Cas9 enzyme unable to cut DNA. “Whereas CRISPR-Cas9 is ...

The CRISPR/Cas9 system consists of Cas9 protein ... targeting activation and gene targeting inhibition tools based on dCas9, called CRISPRa and CRISPRi respectively. In the paper, characteristics ...

In this study, researchers used an epigenome therapy platform based on the CRISPR/dCas9-editing strategy to lower the levels of APOE-e4. The lead candidate from this platform was found to reduce ...

Beyond the gene editing applications enabled by the nuclease CRISPR–Cas9 and CRISPR–Cas12a, the invention of the nuclease-dead Cas molecules (dCas9 and dCas12a) offers a platform for the precise ...

We adapted CRISPR activation using dCas9-VPR to functionally interrogate TTNtv pathogenicity and develop a therapeutic in human cardiomyocytes and 3-dimensional cardiac microtissues engineered from ...

In summary, we report that (I) the CRISPR-nuPin strategy is a two-component platform consisting only of a dCas9-emerin fusion protein and sgRNAs, which is smaller and simpler than most of the ...