The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...

The sequence required immediately downstream of the target sequence. The PAM varies depending on the bacterial origin of the Cas9 protein. DSB: double-strand break. CRISPR/Cas9 introduces a blunt DSB ...

An extensive arsenal of biosensing tools has been developed based on the clustered regularly interspaced short palindromic repeat (CRISPR) platform ... to sensitively detect unique copies of a target ...

The discovery of clustered regularly interspaced short palindromic repeats (CRISPR) DNA segments and CRISPR-associated proteins (Cas), known as CRISPR/Cas, has revolutionized biomedical research ...

No doubt 2025 will usher in many more advances in CRISPR therapies, thanks to the FDA’s new Platform Technology Designation ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

This study presents an optimized CRISPR-Cas12a system for large-scale genetic screening, revealing critical genes involved in ...

This manuscript describes a fundamental investigation of the functioning of Cas9 ... PAM recognition and underscores the importance of fine-tuning the flexibility of the PAM-interacting cleft as a key ...

1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900 USA 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical ...