CRISPR-Nickase leverages the fact that humans have two copies of each gene—one from each parent. Cas9, the molecular scissors that excise target genes, has two active sites that severs each copy.

7 Besides protein engineering, another method to effectively reduce off-target effects is by employing CRISPR nickase to modify one nuclease domain in only one strand of DNA. 6 Unlike normal Cas9 ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into the roles that specific genes play in many diseases. But to date this ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Cas9, the first Cas protein repurposed for cutting DNA, introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, ...