In normal cells, DNA breaks caused by CRISPR–Cas9 are frequently repaired through non-homologous end joining (NHEJ), resulting in unintended insertions or deletions (INDELs).

The CRISPR-Cas9 patent landscape remains complex and unsettled. The Federal Circuit’s latest decision in University of California v. Broad ...

Repair of Cas9-induced DSBs may occur by homologous recombination with the sister chromatid or by accurate or inaccurate nonhomologous end joining (NHEJ). It does not appear that target mutations ...

The Promise of CRISPR-Cas9 CRISPR-Cas9, a revolutionary gene-editing tool, allows for precise modifications in the genome, presenting significant potential in cancer research and treatment.

The CRISPR gene-editing technique has revolutionised biology, but now an even more powerful system called bridge editing could let us completely reshape genomes ...

The incorporation of CRISPR/Cas9 into clinical settings has promising applications for the future treatment of genetic disorders, human immunodeficiency virus (HIV) infection, and various ...

CRISPR-Cas9 gene editing technology is widely used to to help study genes of interest and modify disease-associated genes. However, the system is associated with adverse effects, including ...

While CRISPR/Cas9 is an enormous step forward for plant breeding, it remains nonetheless an expensive and laborious solution, making it unfeasible for application in most plants.

As the originally EGFP to BFP gene editing method was performed by electroporation of plasmids, we first explored gene editing using lipofection of Cas9:gRNA RNP and DNA templates in EGFP-HEK293T ...

During the lack of a repair template, CRISPR/Cas9-induced DNA double-strand breaks (DSBs) are corrected by endogenous cellular DNA repair networks that result in loss-of-function edits.

The CRISPR system, which involves a Cas enzyme to cut DNA, is a powerful tool for gene editing. | Scientists at the University of Texas at Austin have refined the Cas9 protein used in the CRISPR ...

A small molecule could make the CRISPR-Cas9 gene editing tool more efficient, increasing the success of gene insertion experiments and streamlining lab work, according to work by medicinal chemist ...