We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and gene knockout and knockin using CRISPR/Cas9 technologies. 1. STABLE CELL LINE GENERATION ...

A major concern in the applications of the CRISPR/Cas9 system is about its off-target effects ... followed by high-throughput sequencing), IDLVs (integrase defective lentiviral vectors), GUIDE–seq ...

Multiplex CRISPR-Cas9 allows simultaneous editing of several genomic ... allow enhanced expression of the cancer-specific TCR NY-ESO-1, which was introduced by lentiviral transduction. In addition, PD ...

Researchers have revealed new details about the CRISPR-Cas5-HNH/Cascade complex, a variant of the type I-E CRISPR-Cas system, ...

No doubt 2025 will usher in many more advances in CRISPR therapies, thanks to the FDA’s new Platform Technology Designation ...

A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...

CRISPR technology has radically transformed genetic research, and at the forefront of this transformation is CRISPR screening ...

Beans are a key food at the dietary level, boasting high nutritional value and constituting the most directly consumed legume ...

Potent in vivo gene editing in skeletal muscle of non-human primates by a novel, ultracompact CRISPR system delivered via a single AAV vector.