In this study, we demonstrated the exploitation of CRISPR/Cas9 as a high precision system for editing the composition and function of eECMs. Human mesenchymal stromal/stem cell (hMSC) lines were ...

Here’s how it works. CRISPR, short for CRISPR-Cas9, is a genome-editing tool that allows scientists to precisely cut and modify DNA sequences. It has revolutionized the study of genes ...

Because of its precision, ease and adaptability to the needs, the CRISPR-Cas system is now ... clinical trial designs, Figure 3. Western Blot analysis of c-Myc, phospho-cdc2 and p21 expression in MYC ...

However, the cardiac-specific nrp1b knockout significantly ... 15 The CRISPR editing was confirmed by Sanger sequencing and T7E1 mutagenesis assay. CRISPR/Cas9-mediated nrp1a and nrp1b ablation was ...

A western blot, sometimes called a protein immunoblot ... Generating single cell–derived knockout clones in mammalian cells with CRISPR/Cas9. Curr Protoc Mol Biol. 2019;128(1):e100.

In this way, we performed the first CRISPR screen to date in human polarized epithelial cells. Using high-resolution microscopy, we detected polarization defects and mislocalization of DPP4 to late ...

A Double‐strand break (DSB) in the promoter of the ABCB1 gene induced by the CRISPR‐Cas9 lentiviral‐based system leads ... the clone number. C. Western blot showing the levels of the PgP and control ...

Representative western ... experimental CRISPR‐Cas9 (clustered regularly interspaced short palindromic repeats)‐(CRISP‐associated protein 9) approach: Cas‐9 expressing‐RKO cells were transduced with a ...

A comprehensive study headed by researchers at Sanford Burnham Prebys has shown that gene editing—specifically gene knockout (KO)—using CRISPR-Cas9 technology can favor cells with mutated ...

Using genomewide CRISPR knockout screening, we identified the placenta-associated ... and images were taken by a Keyence BZ-X810 fluorescence microscope. For Western blotting, cell were lysed in 1% ...