In this study, we demonstrated the exploitation of CRISPR/Cas9 as a high precision system for editing the composition and function of eECMs. Human mesenchymal stromal/stem cell (hMSC) lines were ...

followed by western blot analysis. The CRISPR/Cas9 system was utilized to generate Tet2 knockout cells. Lentiviruses were produced by co-transfecting HEK293T cells with plasmids containing sgRNA (8μg) ...

Latest study leverages new constructs for nuclear localization signal (NLS) sequences to improve editing efficiency in human ...

In a new development, researchers have successfully created an efficient two-line system for hybrid seed production in ...

Join us for an insightful webinar on the latest advancements in cell and gene therapy, focusing on the innovative Cas9-CLIPT technology. Cas9-CLIPT (Cleaved, Linearized with Protein Template ...

Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City 700000, Vietnam Vietnam National University, Ho Chi Minh City ...

Dsc2 KO and knock-in (KI) mouse models were generated using CRISPR/Cas9 technology on a C57BL/6J genetic background. Dsc2 KO mice were generated by deleting exon 4. KI mice were created by inserting ...

Here, we identify S100PBP as a key modulator of both XY and autosomal recombination in mice. S100pbp-knockout mice exhibit male infertility and spermatogenesis arrest at meiotic metaphase I, resulting ...