DNA bricks for USER assembly were generated by PCR amplification with Phusion U Hot Start DNA polymerase (Thermo Fisher Scientific) and uracil-containing primers (Integrated DNA Technologies, ...

(A) RT-PCR analysis of CP (-) sequence ... for off-target effects with CRISPR/Cas9 technology. The process is time-consuming, requiring multiple breeding generations and thorough molecular screening ...

A first-in-human clinical trial tests a CRISPR/Cas9 gene-editing technique to help the immune system fight advanced gastrointestinal (GI) cancers.

Several human pathogens exploit the kinetic forces generated by polymerizing actin to power their intracellular motility. Human cell-autonomous immune responses activate ...

Achieving reliable PCR and qPCR results is not just about the reagents—consumables play a crucial role too. 4titude® PCR plates from Azenta Life Sciences are optimized to meet the varied and often ...

To address these challenges, we combined genome-scale CRISPR/Cas9 knockout screening, single-cell-CRISPR Purterb RNA sequencing (scRNA-seq), and AI-driven virtual screening to identify novel ...

Knowing the role of exosomes in the human body is important to understanding the impact of exosomal RNA sequencing ... Award Winner in the PCR category. Innovative Products – Hold more than ...

The team of biologists overcame this issue by repurposing CRISPR—a revolutionary gene ... each type of microbe and reading these using DNA sequencing, we can provide a clearer picture of the ...