Scientists from Zhejiang University have developed a novel delivery vehicle for CRISPR-Cas9 that is more effective than lipid nanoparticles (LNPs) in treating lung cancer. Rapid liquid nitrogen ...

They specifically showed this with the protein Cas9. Live Science spoke with Šikšnys about what it's been like to see CRISPR enter clinical use and how he thinks the system might be applied and ...

Schematic of the CRISPR/Cas9 system and its nanotechnology delivery methods. The CRISPR/Cas9 system can be delivered in the forms of DNA, mRNA/sgRNA, or ribonucleoprotein (RNP). The gene editing ...

For a decade, leading academic institutes and their associated companies fought a bruising, headline-grabbing fight over who held patent rights to CRISPR-Cas9, the revolutionary genome editing tool.

Subsequently, DNA repair pathways like non-homologous end joining (NHEJ) or homology-directed repair bring the Cas9 and sgRNA together to ultimately modify the genome. The incorporation of CRISPR ...

The gene editing technique CRISPR/Cas9 has allowed researchers to make precise and impactful changes to an organism's DNA to fix mutations that cause genetic disease. However, the CRISPR/Cas9 ...

Whilst genome editing with CRISPR-Cas9 is effective, it runs the risk of off-target effects and excessive mutations, which can lead to toxicity. To reduce the likelihood of these occurring, ...

CRISPR-Cas9 gene editing technology is widely used to to help study genes of interest and modify disease-associated genes. However, the system is associated with adverse effects, including ...

The CRISPR/Cas9 gene editing system aims to do just that. Researchers have been modifying and improving the technique since it was developed about a decade ago, and it has even been used in the clinic ...

Now, scientists at the University of Texas (UT) at Austin have refined the Cas9 protein used in the Nobel Prize-winning CRISPR-Cas9 tool. The new version, dubbed SuperFi-Cas9, was thousands of ...

CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations. Nature Communications , 2022; 13 (1) DOI: 10.1038/s41467-022-28244-5 Cite ...

More information: Ida Höijer et al, CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations, Nature Communications (2022). DOI ...