This changed with the development of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 ... the Cre recombinase protein. This phage recombination system is the ‘gold standard’ of ...

has approved CASGEVY™ (exagamglogene autotemcel [exa-cel]), a CRISPR/Cas9 gene-edited cell therapy, for the treatment of transfusion-dependent beta thalassemia (TDT) in patients 12 years and older.

While previous methods using viral vectors and genetic manipulation in mice have been time consuming and expensive, here, we describe a technique using cationic polymers as nonviral carriers for ...

These genetically modified mice were created using CRISPR/Cas9 technology on B6D2F1 zygotes. Two distinct methods were employed: microinjection of Single-guide ribonucleic acids (sgRNAs ...

Recent advances in CRISPR ... mouse model containing a Cre recombinase targeted insertion with over 50% knock-in efficiency directly comparing efficiencies to other commonly used approaches. Our ...

The details of the new CRISPR method were outlined in a news release by PNAS NEXUS published on Tuesday. “A study in mice reports a CRISPR/Cas9 gene-editing delivery system, capable of bypassing ...

Note that clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR ... mice for HCM prevention. To this end, they used an AAV9 vector encoded Staphylococcus aureus Cas9.

The gene drive used for this experiment is built on the molecular scissors known as CRISPR/Cas9. Male mosquitoes ... drive to use as a research tool in mice (SN Online: 1/23/19).

Such CRISPR/Cas9-based genome editing strategies traditionally rely on homology-dependent recombination (HDR ... We show that TKIT enables efficient KI in primary cultured neurons from mouse and rat ...