Biofilm was washed with 1X PBS and analyzed using Crystal Violet staining following previously established protocol (She et al ... 2.12.3 N-phenyl naphthylamine (NPN) uptake assay An N-phenyl ...

Subsequently, the simulated semen was used to establish an optimal standard protocol based on the results of the resazurin colorimetric assay. Finally ... Methods to grow and measure in vitro static ...

PCR amplification was conducted in an automated thermal cycler (PTC-200, MJ Research, USA) using the following protocol: initial denaturation ... thiocyanate to sulphate and ammonia during different ...

Biofilms present the next challenge in microbiology — to confront this complexity and devise more relevant testing protocols to deal with demanding microbial problems in industry and medicine.

Conversely, the qualitative CRA method demonstrated comparable results mainly with strong biofilm producers in the SAF assay rendering it efficiently discriminatory for positive-slime phenotypes of S.

Solid-state NMR analysis of Bacillus subtilis biofilms reveals critical phases of maturation and dispersal, impacting medical ...

2015; Mizan et al., 2016). V. parahaemolyticus has the high capacity to adhere to food-contact surfaces (aquaculture equipment, aquatic products, and food processing facilities) and forms biofilm (Han ...

The metabolic output assay therefore becomes quantitative as well as qualitative. Minor changes in growth behaviour, such as metabolic pathway mutations, are detected, as are biofilm ...

Future Microbiol. 2014;9(6):725-735.

Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. Slippery liquid-infused porous surfaces (SLIPSs) are a class of surface that offers ...

1 Division of Molecular and Genetic Medicine, Royal Hallamshire Hospital, Sheffield, UK 2 Gastroenterology and Liver Unit, Royal Hallamshire Hospital, Sheffield, UK 3 Gastroenterology Unit, John ...