Harwood, University of Washington, Seattle, WA, and approved August 10, 2012 (received for review April 27, 2012) It has emerged over the last decade that small noncoding RNAs (sRNAs) have an ...

We developed a novel microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from bacterial cells in the range of 5000 down to a single cell in the sample volume of ...

Therefore a search was made for antagonistic activities against food spoilage and pathogenic bacteria, in isolates from a range of meat and meat products. After purification, the cultures were then ...

Somatic hypermutation (SHM) of immunoglobulin variable (V) regions modulates antibody-antigen affinity is initiated by activation-induced cytidine deaminase (AID) on single-stranded DNA (ssDNA).

Next, viral RNA was extracted from the flaviviruses using the QIAamp viral RNA extraction kit (Qiagen, Germany) and converted to double-stranded complementary DNA (cDNA) using Superscript III reverse ...

Although better binding chemistries and ultra-low input kits promise better extraction, they have to depend on either using large carrier RNA or strong chemicals that can eventually make their way ...