In 1992, graduate student Yasumasa Ishida discovered PD-1, marking the beginning of a journey that would make this molecule a ...

Patients suffering from Parkinson's disease may soon benefit from a powerful treatment option: stem-cell transplants. In a pair of small studies designed primarily to test safety, two teams of ...

We conducted additional annotation on 1134 DEGs and identified that 27 of these genes were associated with the encoding of GPCRs (Supplementary File 2). Among the set of ... University (Guangzhou, ...

stimulation to investigate underlying mechanisms of the activation of lipolysis and autophagy related genes and signaling pathways of adipocytes differentiated from 3T3-L1 cells. LIPUS with the center ...

One of the pathways contributing to the production of cytosolic acetyl-CoA is mediated by acetyl-CoA synthetase 2 (ACSS2). Here ... plasmids were transfected into ACSS2 knockdown 3T3-L1 cells using ...

Stimulation of AMPK by curcumin resulted in the down-regulation of PPAR (peroxisome proliferator-activated receptor)-γ in 3T3-L1 adipocytes and the decrease in COX-2 in MCF-7 cells. Application of a ...

Treatment of 3T3-L1 cells with CAL for 24 h as well as 12 days decreased PPARγ (24 h: −33.3 ± 6.38%; 12 d: −42.1 ± 4.51%), C/EBPα (24 h: −37.5 ± 7.42%; 12 d: −32.6 ± 6.19%), and C/EBPβ (24 h: −22.6 ± ...

The plasmid and Lipofectamine mixture was incubated for 15–20 min at room temperature (RT) and then added to the 3T3-L1 cells. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays ...

Therefore, the present study is aimed to analyze the transcriptomic changes during adipocyte differentiation in a 3T3-L1 cell line ... origin), 2 mM L-glutamine, and 100 U/ml of penicillin and 100 ...