Vacuoles continuously burn adenosine triphosphate (ATP) to drive this process, but once ATP runs low, the vacuoles stall and leech protons back into the cytoplasm. “It’s kind of like a leaky ship ...

Now we have the tools to do that,” he explains, referencing the Calico Labs team’s development of a molecule whose fluorescence level corresponds to the acidity of the yeast vacuole.

These two events may be linked because the yeast vacuole’s membrane contains two complexes of proteins that are important for cell division. When the complexes are far apart, cell division stops.

The terminal tail region of a yeast myosin-V mediates its attachment to vacuole membranes and sites of polarized growth. Proc. Natl Acad. Sci. USA 95 , 14799–14804 (1998) ...

To clarify, macronucleophagy is an autophagy pathway in which a part of the nucleus bulges outwards, is encapsulated by a vesicle called an autophagosome, and is delivered to the vacuole.

Scientists have now defined key events that take place early in the process of cellular aging. Together the discoveries, made through a series of experiments in yeast, bring unprecedented clarity ...

If a daughter yeast cell doesn’t inherit a vacuole, for instance, it will grow the organelle from scratch. Lois Weisman’s team at the University of Michigan hadn’t probed too deeply into this backup ...

But the vacuole of baker's yeast, a widely-studied organism, has a membrane large enough to observe the structural details of each step in the fusion process.

These two events may be linked because the yeast vacuole's membrane contains two complexes of proteins that are important for cell division. When the complexes are far apart, cell division stops.