Before PCR, the process of DNA sequencing was very lengthy and quite expensive. How did the discovery of the Taq polymerase result in this important research technique? To amplify a DNA fragment ...

Henry Erlich, Children’s Hospital Oakland Research Institute Still dedicated to making the technique work, Cetus Corporation scientists David Gelfand and Susanne Stoffel purified a thermostable DNA ...

DNA analysis is crucial for identifying individuals and their relatives, using technologies like PCR, electrophoresis, and fingerprinting.

The technique, now a staple of every molecular biology lab, uses the thermostable Taq DNA polymerase to extend short single-stranded synthetic primers using the target DNA or cDNA as a template ...

The team focused on T7 DNA polymerase’s thioredoxin binding domain (TBD), which has a structure similar to that of most thermostable polymerases, including Taq. Thioredoxin, which is ubiquitous in ...