Figure 6: Transfer of human TRA-YAC into the mouse genome by ES cell–yeast spheroplast fusion. Often, YACs integrate into the mouse genome as a single copy 34. However, one cannot exclude that ...

They can be found naturally and in the laboratory. The name “spheroplast” comes from the circular shape which is adopted by bacterium after removal of its cell wall. This circular shape aids ...

In one approach to identifying the gene functionally, transfer of commercially available yeast artificial chromosomes from the 1q42 region by spheroplast fusion led to identification of a 1.2-Mb ...

This can result in an enrichment of certain proteins compared with preparation by lysis of the entire cell. A spheroplast lysis buffer is also provided for isolation of spheroplast proteins. Never ...