Recombinase-based methods allow scientists to move pieces of DNA from plasmid to plasmid without restriction enzymes, simplifying and expediting the cloning process. It also prevents the aggravation ...

Prepare the restriction enzyme digests. You will perform two single digests using enzymes EcoRI and HindIII and one double digest using both enzymes. You will also be given some plasmid prepared by ...

“I then purified this plasmid cloning vector that had the gal operon and ... Another student in Berg’s lab, John Morrow, had just shown that the restriction enzyme EcoRI (that he had obtained from ...

A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

This process is referred to as cloning. It uses restriction enzymes to cut the plasmid and the gene fragment at sequence-specific locations that depend on the enzyme used. Then, enzymes called ligases ...

Restriction enzymes cut DNA creating complementary ‘sticky ends’ that join by base pairing. A different enzyme is used to join the insulin gene and the bacterial plasmid. The bacterial plasmid ...

Plasmid DNA mini-preps In addition to larger ... and was well digested by restriction enzymes EcoRI and HindIII under standard conditions (data not shown). This technique offers three improvements ...

The Ti plasmid is removed from the agrobacterium cell, and a restriction enzyme cleaves the T-DNA at a specific restriction site. Subsequently, foreign DNA, which has been cleaved by the same enzyme, ...

Prepare plasmid DNA using standard miniprep protocols ... 10 μl of each miniprep with the appropriate pair of restriction enzymes and separating the fragments by electrophoresis through an ...