Nick 3–4 μg of the target plasmid pNL RR2 (or pNL Rep) using either Nt.BbvCI or Nb.BbvCI, depending on the strand to be labeled, for 30–60 min at 37 °C with 2–5 units of enzyme per μg DNA ...

PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction. Nature Communications , 2022; 13 (1) DOI: 10.1038/s41467-022-30355-y Cite This Page : ...

the structure of ‘stabilized plasmid-lipid particles’ (splp) and their properties as systemic gene therapy vectors has been investigated. we show that splp can be visualized employing cryo ...

Enzyme Delivered In Smaller Package Protects Cells From Radiation Damage Date: June 4, 2007 Source: University of Pittsburgh Schools of the Health Sciences ...

Well, you’ll go “ApE” over A Plasmid Editor, a program as simple in use as it is in name. ApE is free to download and runs across all three major platforms: Windows, OS X, and Linux/Unix.

Although plasmid DNA has proven its worth, by enabling production and availability of vaccines, the global demand for DNA, primarily in the form of plasmid DNA, has risen dramatically in recent years.

This requires a ‘ligase’ enzyme and forms a recombinant plasmid. The plasmid is then introduced to bacterial host cells by a heat shock. This step multiples the recombinant vector’s copy number.