In principle, cloning PCR products necessitates some ... insertions or deletions in plasmid DNA. “The use of Phusion Hot Start DNA polymerase in amplification guarantees extreme fidelity and ...

A new study in the journal Human Gene Therapy indicates that DNA impurities derived from plasmid and host cell DNA are ...

The cutting and joining manipulations that underlies gene cloning are carried out by enzymes called restriction ... in order to cut the insert (=the gene) from the plasmid DNA (=vector). Following ...

In step 2, the gene fragment is integrated into a circular piece of DNA, called a plasmid, which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid ...

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To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA (for example, DH10B or XL1-Blue) and plate ...

Service can be initiated at the cloning stage where we clone your transgene into the adenovirus backbone plasmid, at the transfection stage ... Several plaques are selected, expanded, HIRT DNA is ...