Cells are homogenized in guanidinium thiocyanate and the RNA is purified from the lysate by extraction with phenol-chloroform at reduced pH 1. This method allows many samples to be processed ...
Extract the mixture once with phenol/chloroform and once with chloroform. Precipitate the DNA with ethanol in the presence of 0.3 M sodium acetate (pH 5.2) and dissolve in 90 μl of Tris/EDTA (TE ...
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