Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a ...

Note, the pH is 9.2 (the optimal for long PCR) without adjustment; some vectors may require pH 7.2. 10-40 dNTP mix: 10 mM each dNTP, 40 mM MgCl 2; dissolve the dry form of each dNTP in water to ...