a pre-amplification step using primers that match the adapter sequences and that carry each one selective nucleotide (represented by N) at their 3 end are used to PCR-amplify subsets of the EcoRI ...

Figure 1: Amplification of complex gene libraries by conventional PCR and emulsion PCR. Figure 2: Ethidium bromide–stained agarose gels reveal the absence of size competition and chimeric produc ...