ChVLig encircles the DNA as a C-shaped protein clamp. The NTase domain binds to the broken and intact DNA strands in the major groove flanking the nick and also in the minor groove on the 3’-OH side ...

The third α-helix, which is the second or recognition helix of the helix-turn-helix, is docked into the major groove roughly perpendicularly to the helical axis of the DNA. The DBD makes ...

This first loop sits in the major groove and makes sequence-specific contacts for all six proteins. The number of contacts, and therefore the overall contribution to DNA binding specificity is ...