The choice of label is based upon the signal-to-noise ratio of the label, the capabilities of the flow cytometer that will be used for the assay ... success of the tetramer production is the binding ...

Containing mutations in the MHC α3 domain that ablate CD8 binding; can be used to quantitate and to sort ... have affinities that are too low for target detection under normal assay conditions, and ...

The yield from a tetramer preparation is variable, but typically the product is sufficient for hundreds of stainings, often thousands of stainings. The stability of MHC class I tetramers is variable ...

Recently developed major histocompatibility complex (MHC) multimer technologies allow ... complete and very fast competitive binding of d-biotin is achieved even at low temperatures 22; second ...

In A, cellular peptide binding assays of the wild-type p11C peptide and the mutant peptide (p11C*) for the MHC class i molecule Mamu-a*01 show reduced recognition for the mutant. The p11B peptide ...

How many naive T cells that are specific for any one peptide–MHC complex exist in ... fluorochrome component of the tetramer. This allowed all the tetramer-binding cells to be concentrated ...