Figure 1: β-galactosidase expression in mouse liver following lentiviral transduction. We analysed liver toxicity in rodents by measuring the serum alanine aminotransferase (ALT) concentration ...

To improve the efficacy of gene therapy when using anti-sickling beta globin gene transfer, they added cyclosporin (CsH) to increase transduction by inhibiting lentiviral restriction factors.

Viral gene delivery is a ubiquitous laboratory technique with a myraid of uses. Understanding the basics of gene delivery workflows, from vector selection and cloning to transduction, is a fundamental ...

Lentiviruses have the unique ability amongst retroviruses of being able to infect non-cycling cells. Vectors derived from lentiviruses have provided a huge advancement in technology and seemingly ...

16 For simultaneous double lentiviral transduction, cultures divided beyond p50, except that ipnNF09.4 was only taken to p47, but did not show signs of senscence at that point, and sipnNF95.12B ...

Here we asked if increasing NaCl tonicity in the vector formulation enhances the transduction efficiency of 3 gene therapy vectors: adenovirus, AAV, and lentiviral vectors. We observed that vectors ...

We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and gene knockout and knockin using CRISPR/Cas9 technologies. 1. STABLE CELL LINE GENERATION ...

More recently, the Vector Development Laboratory merged with our group, and we have expanded our services to offer AAV, first-generation adenovirus (FGAd), helper-dependent adenovirus (HDAd), ...