Lentiviral transduction is a popular and powerful gene delivery method for downstream applications such as cell line generation, therapeutic model generation and CAR-T research. However, current ...

We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and gene knockout and knockin using CRISPR/Cas9 technologies. 1. STABLE CELL LINE GENERATION ...

A team of CiRA researchers has uncovered the crucial role of EIF3D—a protein translational regulator—in primed pluripotency.

This study “harnesses CRISPR pooled screening technology to help us figure out where the functional regions in the noncoding genome [are],” he explained. Gersbach and colleagues created lentiviral ...

CRISPR screening is an application of this technology, and we can do the screens in two ways. Our pooled format takes huge numbers of lentiviral guide RNAs and delivers them to a large population ...

Viral gene delivery is a ubiquitous laboratory technique with a myraid of uses. Understanding the basics of gene delivery workflows, from vector selection and cloning to transduction, is a fundamental ...

CRISPR interference (CRISPRi ... The guide associates with dCas9-SALL1-SDS3 and directs the complex to the DNA target site. Horizon offers lentiviral and transient approaches for both repressor ...