To improve the efficacy of gene therapy when using anti-sickling beta globin gene transfer, they added cyclosporin (CsH) to increase transduction by inhibiting lentiviral restriction factors.

We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and gene knockout and knockin using CRISPR/Cas9 technologies. 1. STABLE CELL LINE GENERATION ...

A team of CiRA researchers has uncovered the crucial role of EIF3D—a protein translational regulator—in primed pluripotency.

This study “harnesses CRISPR pooled screening technology to help us figure out where the functional regions in the noncoding genome [are],” he explained. Gersbach and colleagues created lentiviral ...

Viral gene delivery is a ubiquitous laboratory technique with a myraid of uses. Understanding the basics of gene delivery workflows, from vector selection and cloning to transduction, is a fundamental ...

CRISPR interference (CRISPRi ... The guide associates with dCas9-SALL1-SDS3 and directs the complex to the DNA target site. Horizon offers lentiviral and transient approaches for both repressor ...

Here we asked if increasing NaCl tonicity in the vector formulation enhances the transduction efficiency of 3 gene therapy vectors: adenovirus, AAV, and lentiviral vectors. We observed that vectors ...