Lentiviral transduction is a popular delivery platform for applications such as CAR-T cell engineering or CRISPR-Cas9 gene editing, as it can transduce both dividing and non-dividing cells and ...

researchers are more frequently selecting lentiviral transduction because (1) it provides long-lasting gene expression, (2) it is compatible with knock-in, knock-out, and knock-down CRISPR ...

However, CRISPR-Cas9 gene editing in primary T cells, a key target for gene-engineering approaches, often results in low transduction and variable gene-editing efficiency. To date, lentiviral vectors ...

CRISPR is transforming the treatment of ... in this study was performed using lentiviral transduction. Lentiviral insertion of transgenes has been the dominant method in engineering T cells ...

Lentiviral transduction is a popular and powerful gene delivery method for downstream applications such as cell line generation, therapeutic model generation and CAR-T research. However, current ...

Cytidine deamination that is guided by clustered regularly interspaced short palindromic repeats (CRISPR ... cytometry after base editing and lentiviral transduction with high-level disruption ...

To improve the efficacy of gene therapy when using anti-sickling beta globin gene transfer, they added cyclosporin (CsH) to increase transduction by inhibiting lentiviral restriction factors.

Autologous HSCs were transduced with CD68-ET3-LV — a lentiviral vector including a new F8 transgene (ET3) with a myeloid-directed CD68 promoter — either without transduction enhancer (group 1 ...

such as lipid nanoparticles and lentiviral vectors, but it remains a key challenge. Some biotech companies are developing strategies that use CRISPR to target the DNA of certain bacterial species.