Study: Re-formation of synaptic connectivity in dissociated human stem cell-derived retinal organoid cultures.Image Credit: Kateryna Kon/Shutterstock Background. Synapse-forming retinal cells ...

Ludwig AL, et al. Re-formation of synaptic connectivity in dissociated human stem cell-derived retinal organoid cultures. PNAS. 2023;120(2):e2213418120. Beier KT. The serendipity of viral ...

That process produces actual retinal neurons organized as complex tissue, without any external stimulation of development pathways during specialization. The method has its limitations, though.

My team has developed a method to transplant retinal sheets into degenerating retinas. 1 Because the retina has a very complex structure, it would be very difficult to get the photoreceptors to ...

The researchers found that the modified rabies virus traversed across the synapse to stain all three major retinal cell types 20 days after removal from the retinal organoid.

Using deep Hi-C sequencing, a tool used for studying 3D genome organization, the researchers created a high-resolution map of chromatin in a human retinal organoid at five key points in development.

"We confirmed for the first time in a non-human primate model that embryonic stem-derived retinal organoid sheet transplantation facilitates the closure of macular holes," says senior study author ...

Cross-section through part of a retinal organoid: On day 143 of their differentiation, the inner nuclear layer (bottom) contains the cell bodies of functionally distinct cells: those of the ...

Using immunocytochemistry techniques, they characterized proteins associated with specific retinal cells present at ...

For example, organoid protocols enable the generation of RGCs that more closely resemble those found in the human retina, thus improving the accuracy and efficacy of transplantation.