Another challenge is that CRISPR doesn’t always achieve a complete knockout of the gene targeted in both copies of the gene. If even one copy is still functioning, the phenotype-genotype match ...

In the few past years, engineered endonucleases (Meganucleases, ZFN, TALENS) and CRISPR/Cas9 technology have revolutionized genome editing possibilities. Genome editing can now be used in a wide range ...

Most commonly, CRISPR knockout is used, but CRISPR activation ... providing a clearer picture of the relationship between the genotype and phenotype. A third option is single-cell CRISPR screening.

The rapid adoption and ease of use of CRISPR-Cas9 has driven the development of applications for gene knockout, precise gene editing, and transcriptional regulation. This allows researchers to more ...

Aconsortium of 17 laboratories worldwide has presented results contradicting a highly cited study that described a technique to create conditional knockout mice using CRISPR. The preprint, published ...

CRISPR-based genome editing has accelerated biological research and holds great potential for studying and treating human diseases. The CRISPR-Cas9 system requires a Cas9 nuclease and a guide RNA, ...

Led by Aaron Gitler, Ph.D., professor of genetics, and Michael Bassik, Ph.D., assistant professor of genetics, the team applied CRISPR/Cas9 knockout (KO) screens in human cells and in primary ...