Two novel CRISPR-Cas12a libraries, named Menuetto and Scherzo, were created to perform large-scale genetic knockout screens. The Menuetto library was designed to enhance target coverage ...

This may prevent current knockout models from accurately replicating the subtle changes in function associated with these ...

There is general appreciation that 'genetic background effects' can profoundly affect the immune phenotypes of congenic, transgenic and knockout mice. We suggest that attributing phenotypes to ...