Chalfie thought that if GFP could be taken out of these marine organisms and inserted into other cells, it could act as a reporter in a living animal. However, nobody knew if this would be possible.
while Tsien saw long-term potential in making GFP glow brighter so it could be used as an all-purpose biological reporter. In the end, GFP proved to be a stand-alone structure, tailor-made for the ...
This was ingeniously exploited, and GFP was used as a reporter for the folding of upstream fusion proteins in E. coli. Our findings demonstrate that fusion of GFP to amino acidic tags also affects ...
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