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EGFP was fluorescently quantified on a DeNovix QFX fluorometer using the BioVision GFP Quantitation Kit. A 200 μL total assay volume was prepared in 0.5 mL, thin-wall PCR tubes. To make 1 μg/μL ...
Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants
This assay involves attaching MTs to a coverslip and then adding low levels of GFP-tagged kinesin to ... was continuously measured in an Eppendorf Spectrophotometer (UV-Vis BioSpectrometer ...
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