Here we present two possible protocols describing the design and cloning of silencing lentiviral vectors. These protocols can be completed in less than 3 weeks. ... For example, effective ...

VDL SOPs: As a service organization, we believe it will benefit you to have access to the Standard Operating Procedures we use to create, expand, purify, and evaluate your vectors. An SOP for each ...

To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA (for example, DH10B or XL1-Blue) and plate ...