We next aimed to verify the dCas9-EGFP signals with FISH using probes targeting repetitive sequences so that a single FISH probe could access many sites. We used another two sgRNA sequences ...

The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...

CRISPR-dCas9 and sgRNA – RNA origami fusion molecules Researchers from Aarhus University publish on RNA origami enabling applications in synthetic biology Original Source ...

With a multidisciplinary team in his laboratory at the University of California, San Diego, he now develops tools through genetic engineering techniques such as CRISPR to solve the ... version of Cas9 ...

(Courtesy of Stanley Qi) CRISPRKit works in a similar way, though it employs dCas9, or “dead” Cas9, a deactivated version of the Cas9 enzyme unable to cut DNA. “Whereas CRISPR-Cas9 is ...

It's CRISPR, without the cut. The Horizon CRISPRi system uses a deactivated Cas9 nuclease (dCas9) fused to our proprietary SALL1-SDS3 repressor construct to block transcription of target genes, ...

The researchers wanted to test if they could reverse these changes using epigenomic editing. So, they employed the CRISPR-dCas9 system. Like its widely used sister system CRISPR-Cas9, CRISPR-dCas9 ...