A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

No doubt 2025 will usher in many more advances in CRISPR therapies, thanks to the FDA’s new Platform Technology Designation ...

In particular, we have identified base-specific recognition of the PAM sequence and the positioning of ... crystal structure of the complex formed by anti-CRISPR protein AcrIIA4 and sgRNA-bound type ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it in its search. When the CRISPR Cas9 protein is added to ...

Mammalian cell gene editing has extensively used RNA-guided CRISPR-Cas systems ... showed that it could identify the WTN (W = T or A) PAM sequence, greatly expanding its targeting range.

Traditionally, CRISPR-Cas9 has been the predominant system for gene editing, but limitations such as sequence constraints and off-target effects have driven the search for alternative tools.