A temporally resolved CRISPR-Cas9 screen reveals DAC-induced DNA damage drives trans-cell cycle cytotoxicity that depends on ...

The standard Cas9 protein cuts the DNA at the target. When the cut is repaired, mutations are introduced that usually disable a gene. This is by far the most common use of CRISPR. It’s called ...

A CRISPR-based system that erases itself from fly populations may offer a safer approach to tackling insecticide resistance ...

Her Doudna Lab team at Berkeley has also corrected the genetic mutation that causes sickle cell disease using CRISPR-Cas9. Some scientists warn about the ethical dangers of using gene editing in ...

CRISPR technology has radically transformed genetic research, and at the forefront of this transformation is CRISPR screening ...

Scientists introduced CRISPR to the world as a gene ... treated are removed from the patient's body, then Cas9 tool is applied to correct the mutation — or actually, trigger production of ...

In about a third of patients, the gene for dystrophin has small deletions or single base mutations that render it nonfunctional, which makes this gene an excellent candidate for gene editing.

Analysis of CRISPR/Cas9 edited cells is done to check the efficacy of the system in introducing mutations (insertions, deletions, or substitutions) in the DNA sequences of the edited cell population.

Africa is grappling with severe food insecurity in the wake of consecutive droughts, climate change, and ongoing conflicts, which are affecting millions across the continent.

This study presents an optimized CRISPR-Cas12a system for large-scale genetic screening, revealing critical genes involved in ...