Genetically engineered mouse models that employ site-specific recombinase technology are important tools for cancer research but can be costly and time-consuming. The CRISPR-Cas9 system has been ...

“These CRISPR/Cas9 knockin mice have Cre recombinase-dependent expression of CRISPR associated protein 9 (cas9) endonuclease and EGFP from the mouse endogenous Gt(ROSA)26Sor locus,” the page ...

One of the systems used for conditional gene inactivation is Cre/lox. "It is the gold standard for the conditional gene inactivation in mice but ... system with the CRISPR/Cas9 genetic scissors.

CRISPR technology allows you to generate new mutant mice quickly and precisely. Using the classic CRISPR approach in mouse embryos, single or multiple guide RNAs direct the Cas9 protein to clip ...

More information: Krzysztof Chylinski et al. CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci, Nature Communications (2019). DOI: 10.1038/s41467-019 ...

The researchers demonstrate that this technique can be used in mice to treat several different diseases. Much of the enthusiasm around gene-editing techniques, particularly the CRISPR-Cas9 ...

Scientists have used CRISPR-Cas9 gene editing to lessen some autism symptoms in mice with a form of fragile X syndrome, the most common known single-gene cause of autism spectrum disorder. Employing ...

A new twist on gene editing makes the CRISPR/Cas9 molecular scissors ... In the diabetes experiment, they could use mice already engineered to make their own Cas9. But people don’t naturally ...

Other studies have reported that the mouse immune system can mount a response to Cas9, which could potentially interfere with the benefit of CRISPR therapies. Several groups have also reported ...

to inject the CRISPR-Cas9 gene editing system into living mice; once inside, it was able to snip away one of the genetic mutations thought to be responsible for DMD. Afterwards, these injected ...

This will allow quicker and easier gene knock-down experiments, for example, because having on-board Cas9 makes more room in vectors used to deliver the guide RNA to contain multiple instructions for ...